• Immunofluorescence analysis of U-2 OS cells using HIBCH antibody (STJ11102854) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using HIBCH antibody (STJ11102854) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using HIBCH Rabbit polyclonal antibody (STJ11102854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HIBCH antibody (33-330) (STJ11102854)

SKU:
STJ11102854

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HIBCH (33-330) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIBCH
Gene ID: 26275
Uniprot ID: HIBCH_HUMAN
Immunogen Region: 33-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-330 of human HIBCH (NP_055177.2).
Immunogen Sequence: DAAEEVLLEKKGCTGVITLN RPKFLNALTLNMIRQIYPQL KKWEQDPETFLIIIKGAGGK AFCAGGDIRVISEAEKAKQK IAPVFFREEYMLNNAVGSCQ KPYVALIHGITMGGGVGLSV HGQFRVATEKCLFAMPETAI GLFPDVGGGYFLPRLQGKLG YFLALTGFRLKGRDVYRAGI ATHFVDSEKLAMLEEDLLAL KSPSKENIASVLENYHTESK IDRDKSFILEEHMDKINSC
Tissue Specificity Highly expressed in liver and kidney, also detected in heart, muscle and brain (at protein level). Not detected in lung.
Function Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.
Protein Name 3-Hydroxyisobutyryl-Coa Hydrolase - Mitochondrial
3-Hydroxyisobutyryl-Coenzyme A Hydrolase
Hib-Coa Hydrolase
Hibyl-Coa-H
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-3-Hydroxyisobutyryl-Coa Hydrolase - Mitochondrial antibody
Anti-3-Hydroxyisobutyryl-Coenzyme A Hydrolase antibody
Anti-Hib-Coa Hydrolase antibody
Anti-Hibyl-Coa-H antibody
Anti-HIBCH antibody

Information sourced from Uniprot.org

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