| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human/Rat Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-HGS (C-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | HGS |
| Gene ID: | 9146 |
| Uniprot ID: | HGS_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | Specific for HGS. |
| Immunogen: | A synthetic peptide from the c-terminal region of mouse HGS (Hepatocyte growth factor-regulated tyrosine kinase substrate) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in human and rat. |
| Post Translational Modifications | Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected. Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated. Ubiquitinated by ITCH. |
| Function | Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. |
| Protein Name | Hepatocyte Growth Factor-Regulated Tyrosine Kinase SubstrateHrsProtein Pp110 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-432720Reactome: R-HSA-5689880Reactome: R-HSA-6807004Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8875360Reactome: R-HSA-9013420Reactome: R-HSA-917729Reactome: R-HSA-9635644Reactome: R-HSA-9636383Reactome: R-HSA-9706019 |
| Cellular Localisation | CytoplasmEarly Endosome MembranePeripheral Membrane ProteinCytoplasmic SideEndosomeMultivesicular Body MembraneColocalizes With Ubqln1 In Ubiquitin-Rich Cytoplasmic Aggregates That Are Not Endocytic Compartments |
| Alternative Antibody Names | Anti-Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate antibodyAnti-Hrs antibodyAnti-Protein Pp110 antibodyAnti-HGS antibodyAnti-HRS antibody |
Information sourced from Uniprot.org
