| Host: | Rabbit |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-HGF (106-728) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HGF |
| Gene ID: | 3082 |
| Uniprot ID: | HGF_HUMAN |
| Immunogen Region: | 106-728 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 106-728 of human HGF (NP_000592.3). |
| Immunogen Sequence: | SGVKKEFGHEFDLYENKDYI RNCIIGKGRSYKGTVSITKS GIKCQPWSSMIPHEH |
| Post Translational Modifications | The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form. |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation. |
| Protein Name | Hepatocyte Growth FactorHepatopoietin-AScatter FactorSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1257604Reactome: R-HSA-1266695Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6806942Reactome: R-HSA-6807004Reactome: R-HSA-6811558Reactome: R-HSA-8851805Reactome: R-HSA-8851907Reactome: R-HSA-8865999Reactome: R-HSA-8874081Reactome: R-HSA-8875513Reactome: R-HSA-8875555Reactome: R-HSA-8875656Reactome: R-HSA-8875791Reactome: R-HSA-9734091 |
| Alternative Antibody Names | Anti-Hepatocyte Growth Factor antibodyAnti-Hepatopoietin-A antibodyAnti-Scatter Factor antibodyAnti-Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain antibodyAnti-HGF antibodyAnti-HPTA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89714/358196/STJ11100778_1__20770.1713122514.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")