• HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MYF6 with MYC tag probed with STJ72206 (0. 5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ72206 (0. 5mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-Herculin/MYF6 antibody (Internal) (STJ72206)

SKU:
STJ72206-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB-Trf
Reactivity: Human/Cow/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Herculin/MYF6 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: In transfected HEK293 transiently expressing full-length Human MYF6 (myc and DYKDDDDK tagged) , a band of approx. 37kDa was observed. No bands were observed in mock-transfected HEK293
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MYF6
Gene ID: 4618
Uniprot ID: MYF6_HUMAN
Immunogen Region: Internal
Accession Number: NP_002460.1
Immunogen Sequence: SYRPKQENLEGAD
Function Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Protein Name Myogenic Factor 6
Myf-6
Class C Basic Helix-Loop-Helix Protein 4
Bhlhc4
Muscle-Specific Regulatory Factor 4
Database Links Reactome: R-HSA-525793
Reactome: R-HSA-9839394
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Myogenic Factor 6 antibody
Anti-Myf-6 antibody
Anti-Class C Basic Helix-Loop-Helix Protein 4 antibody
Anti-Bhlhc4 antibody
Anti-Muscle-Specific Regulatory Factor 4 antibody
Anti-MYF6 antibody
Anti-BHLHC4 antibody
Anti-MRF4 antibody

Information sourced from Uniprot.org

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