• Immunofluorescence analysis of L929 cells using HDLBP Rabbit polyclonal antibody (STJ116291) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HDLBP in paraffin-embedded mouse liver using HDLBP Rabbit polyclonal antibody (STJ116291) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HDLBP in paraffin-embedded human liver using HDLBP Rabbit polyclonal antibody (STJ116291) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HDLBP in paraffin-embedded rat liver using HDLBP Rabbit polyclonal antibody (STJ116291) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HDLBP Rabbit polyclonal antibody (STJ116291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HDLBP antibody (1035-1268) (STJ116291)

SKU:
STJ116291

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HDLBP (1035-1268) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDLBP
Gene ID: 3069
Uniprot ID: VIGLN_HUMAN
Immunogen Region: 1035-1268
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1268 of human HDLBP (NP_976221.1).
Immunogen Sequence: LERVKELQAEQEDRALRSFK LSVTVDPKYHPKIIGRKGAV ITQIRLEHDVNIQFPDKDDG NQPQDQITITGYEKNTEAAR DAILRIVGELEQMVSEDVPL DHRVHARIIGARGKAIRKIM DEFKVDIRFPQSGAPDPNCV TVTGLPENVEEAIDHILNLE EEYLADVVDSEALQVYMKPP AHEEAKAPSRGFVVRDAPWT ASSSEKAPDMSSSEEFPSFG AQVAPKTLPWGPKR
Function Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.
Protein Name Vigilin
High Density Lipoprotein-Binding Protein
Hdl-Binding Protein
Database Links Reactome: R-HSA-8964011
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Vigilin antibody
Anti-High Density Lipoprotein-Binding Protein antibody
Anti-Hdl-Binding Protein antibody
Anti-HDLBP antibody
Anti-HBP antibody
Anti-VGL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance