| Short Description : | Mouse monoclonal anti-HCV subtype 1b NS5B protein (77-86 aa) for use in ELISA, WB and IP in Human and HCV samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/WB/IP |
| Host: | Mouse |
| Reactivity: | Human/HCV |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 7F12 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Purification: | Protein G purification |
| Dilution Range: | WB in reduced conditions 1 µg/ml; Optimal conditions for IP should be determined for each particular application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Formulation: | PBS pH 7.4, with 0.1% sodium azide |
| Storage Instruction: | Store at-20 to-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Immunogen: | Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp). Mapped to amino acids 77-86 (TVKAKLLSVE) |
| Immunogen Region: | 77-86 aa |
| Specificity: | Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 77-86 (TVKAKLLSVE) |
| Background | Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. |
Information sourced from Uniprot.org