Host: |
Mouse |
Applications: |
ELISA/WB/IP/IF |
Reactivity: |
Human/HCV |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Mouse monoclonal antibody anti-HCV subtype 1b NS5B protein (111-130 aa) is suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence research applications. |
Clonality: |
Monoclonal |
Clone ID: |
9A2 |
Conjugation: |
Unconjugated |
Isotype: |
IgG1 |
Formulation: |
PBS pH 7.4, with 0.1% sodium azide |
Purification: |
Protein G purification |
Dilution Range: |
WB in reduced conditions 1 µg/ml; IF 2 µg/mlOptimal conditions for IP should be determined for each particular application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
Storage Instruction: |
Store at-20 to-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
Immunogen Region: |
111-130 aa |
Specificity: |
Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT) |
Immunogen: |
Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp). Mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT) |
Background | Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance