• Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Nucleocapsid Protein (His Tag) , using HCoV-OC43 Nucleoprotein Rabbit polyclonal antibody (STJ11103401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HCoV-OC43 Nucleoprotein antibody (100-200) (STJ11103401)

SKU:
STJ11103401

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Host: Rabbit
Applications: WB
Reactivity: HCoV-OC43
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCoV-OC43 Nucleoprotein (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of coronavirus Nucleoprotein (YP_009555245.1).
Immunogen Sequence: YWYRHNRRSFKTADGNQRQL LPRWYFYYLGTGPHAKDQYG TDIDGVYWVASNQADVNTPA DIVDRDPSSDEAIPTRFPPG TVLPQGYYIEGSGRSAPNSR S
Background Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV) , which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Information sourced from Uniprot.org

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