• Western blot analysis of lysates from Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECDHis Tag) , using HCoV-NL63 Spike S2 Rabbit polyclonal antibody (STJ11103402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HCoV-NL63 Spike S2 antibody (1200-1300) (STJ11103402)

SKU:
STJ11103402

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Host: Rabbit
Applications: WB
Reactivity: HCoV-NL63
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCoV-NL63 Spike S2 (1200-1300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1200-1300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of coronavirus Spike S2 (YP_003767.1).
Immunogen Sequence: VNISRVELHTVIPDYVDVNK TLQEFAQNLPKYVKPNFDLT PFNLTYLNLSSELKQLEAKT ASLFQTTVELQGLIDQINST YVDLKLLNRFENYIKWPWWV W
Background The protein encoded by this gene is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

Information sourced from Uniprot.org

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