• Western blot analysis of lysates from Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag) , using HCoV-HKU1 Spike S2 Rabbit polyclonal antibody (STJ11103405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HCoV-HKU1 Spike S2 antibody (800-900) (STJ11103405)

SKU:
STJ11103405

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Host: Rabbit
Applications: WB
Reactivity: HCoV-HKU1
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCoV-HKU1 Spike S2 (800-900) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 800-900
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 800-900 of coronavirus Spike S2 (YP_173238.1).
Immunogen Sequence: IVGQEEFIQTNSPKVTIDCS LFVCSNYAACHDLLSEYGTF CDNINSILDEVNGLLDTTQL HVADTLMQGVTLSSNLNTNL HFDVDNINFKSLVGCLGPHC G
Background This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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