• Immunofluorescence analysis of A549 cells using HCK antibody (STJ23924). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of HL-60 cells, using HCK antibody (STJ23924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-HCK antibody (1-220) (STJ23924)

SKU:
STJ23924

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCK (1-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HCK
Gene ID: 3055
Uniprot ID: HCK_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human HCK (NP_001165600.1).
Immunogen Sequence: MGCMKSKFLQVGGNTFSKTE TSASPHCPVYVPDPTSTIKP GPNSHNSNTPGIREAGSEDI IVVALYDYEAIHHEDLSFQK GDQMVVLEESGEWWKARSLA TRKEGYIPSNYVARVDSLET EEWFFKGISRKDAERQLLAP GNMLGSFMIRDSETTKGSYS LSVRDYDPRQGDTVKHYKIR TLDNGGFYISPRSTFSTLQE LVDHYKKGNDGLCQKLSVPC
Tissue Specificity Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
Post Translational Modifications Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases. Ubiquitinated by CBL, leading to its degradation via the proteasome. Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.
Function Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
Protein Name Tyrosine-Protein Kinase Hck
Hematopoietic Cell Kinase
Hemopoietic Cell Kinase
P59-Hck/P60-Hck
P59hck
P61hck
Database Links Reactome: R-HSA-164944
Reactome: R-HSA-2029481
Reactome: R-HSA-912631
Reactome: R-HSA-9664323
Reactome: R-HSA-9664422
Reactome: R-HSA-9674555
Reactome: R-HSA-9680350
Reactome: R-HSA-9705462
Reactome: R-HSA-9706374
Cellular Localisation Isoform 1: Lysosome
Membrane
Lipid-Anchor
Cell Projection
Podosome Membrane
Cytoplasm
Cytosol
Associated With Specialized Secretory Lysosomes Called Azurophil Granules
At Least Half Of This Isoform Is Found In The Cytoplasm
Some Of This Fraction Is Myristoylated
Isoform 2: Cell Membrane
Caveola
Cell Junction
Focal Adhesion
Cytoskeleton
Golgi Apparatus
Cytoplasmic Vesicle
Lysosome
Nucleus
20% Of This Isoform Is Associated With Caveolae
Localization At The Cell Membrane And At Caveolae Requires Palmitoylation At Cys-3
Colocalizes With The Actin Cytoskeleton At Focal Adhesions
Secretory Vesicle
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Hck antibody
Anti-Hematopoietic Cell Kinase antibody
Anti-Hemopoietic Cell Kinase antibody
Anti-P59-Hck/P60-Hck antibody
Anti-P59hck antibody
Anti-P61hck antibody
Anti-HCK antibody

Information sourced from Uniprot.org

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