Western blot analysis of lysates from Rat liver, using Hemoglobin subunit alpha (HBA1) (HBA1) Rabbit mAb (STJ11102472) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HBA1 antibody (1-100) [S2MR] (STJ11102472)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102472
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General Information

Short DescriptionRabbit monoclonal Hemoglobin subunit alpha (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS2MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:6000
IHC-P:1:200-1:2000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolHBA1.HBA2
Gene ID3039/3040
Uniprot IDHBA_HUMAN
Immunogen Region1-100
Immunogen SequenceMVLSPADKTNVKAAWGKVGA HAGEYGAEALERMFLSFPTT KTYFPHFDLSHGSAQVKGHG KKVADALTNAVAHVDDMPNA LSALSDLHAHKLRVDPVNFK
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hemoglobin subunit alpha (HBA1) (HBA1) (P69905).

Additional Info

Tissue Specificity Red blood cells.
Post Translational Modifications The initiator Met is not cleaved in variant Thionville and is acetylated.
Function Involved in oxygen transport from the lung to the various peripheral tissues. Hemopressin: Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.
Protein Name Hemoglobin Subunit Alpha
Alpha-Globin
Hemoglobin Alpha Chain Cleaved Into - Hemopressin
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-2168880
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Cellular Localisation
Alternative Antibody Names Anti-Hemoglobin Subunit Alpha antibody
Anti-Alpha-Globin antibody
Anti-Hemoglobin Alpha Chain Cleaved Into - Hemopressin antibody
Anti-HBA1.HBA2 antibody

Information sourced from Uniprot.org

Citations

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