• Immunofluorescence analysis of C6 cells using HAL Rabbit polyclonal antibody (STJ114988) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HAL in paraffin-embedded mouse heart using HAL Rabbit polyclonal antibody (STJ114988) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HAL in paraffin-embedded human thyroid cancer using HAL Rabbit polyclonal antibody (STJ114988) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HAL in paraffin-embedded human lung cancer using HAL Rabbit polyclonal antibody (STJ114988) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HAL in paraffin-embedded rat kidney using HAL Rabbit polyclonal antibody (STJ114988) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HAL Rabbit polyclonal antibody (STJ114988) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-HAL antibody (1-200) (STJ114988)

SKU:
STJ114988

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAL (1-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAL
Gene ID: 3034
Uniprot ID: HUTH_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).
Immunogen Sequence: MPRYTVHVRGEWLAVPCQDA QLTVGWLGREAVRRYIKNKP DNGGFTSVDDAHFLVRRCKG LGLLDNEDRLEVALENNEFV EVVIEGDAMSPDFIPSQPEG VYLYSKYREPEKYIELDGDR LTTEDLVNLGKGRYKIKLTP TAEKRVQKSREVIDSIIKEK TVVYGITTGFGKFARTVIPI NKLQELQVNLVRSHSSGVGK
Post Translational Modifications Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.
Protein Name Histidine Ammonia-Lyase
Histidase
Database Links Reactome: R-HSA-70921
Alternative Antibody Names Anti-Histidine Ammonia-Lyase antibody
Anti-Histidase antibody
Anti-HAL antibody
Anti-HIS antibody

Information sourced from Uniprot.org

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