• Immunohistochemistry analysis of paraffin-embedded mouse brain using HADH antibody (STJ23909) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using HADH antibody (STJ23909) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spinal cord using HADH antibody (STJ23909) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HADH antibody (STJ23909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HADH antibody (1-314) (STJ23909)

SKU:
STJ23909

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HADH (1-314) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HADH
Gene ID: 3033
Uniprot ID: HCDH_HUMAN
Immunogen Region: 1-314
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human HADH (NP_005318.3).
Immunogen Sequence: MAFVTRQFMRSVSSSSTASA SAKKIIVKHVTVIGGGLMGA GIAQVAAATGHTVVLVDQTE DILAKSKKGIEESLRKVAKK KFAENPKAGDEFVEKTLSTI ATSTDAASVVHSTDLVVEAI VENLKVKNELFKRLDKFAAE HTIFASNTSSLQITSIANAT TRQDRFAGLHFFNPVPVMKL VEVIKTPMTSQKTFESLVDF SKALGKHPVSCKDTPGFIVN RLLVPYLMEAIRLYERGDA
Tissue Specificity Expressed in liver, kidney, pancreas, heart and skeletal muscle.
Post Translational Modifications Succinylation at Lys-81, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5.
Function Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion.
Protein Name Hydroxyacyl-Coenzyme A Dehydrogenase - Mitochondrial
Hcdh
Medium And Short-Chain L-3-Hydroxyacyl-Coenzyme A Dehydrogenase
Short-Chain 3-Hydroxyacyl-Coa Dehydrogenase
Database Links Reactome: R-HSA-77310
Reactome: R-HSA-77346
Reactome: R-HSA-77348
Reactome: R-HSA-77350
Reactome: R-HSA-77352
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Hydroxyacyl-Coenzyme A Dehydrogenase - Mitochondrial antibody
Anti-Hcdh antibody
Anti-Medium And Short-Chain L-3-Hydroxyacyl-Coenzyme A Dehydrogenase antibody
Anti-Short-Chain 3-Hydroxyacyl-Coa Dehydrogenase antibody
Anti-HADH antibody
Anti-HAD antibody
Anti-HAD1 antibody
Anti-HADHSC antibody
Anti-SCHAD antibody

Information sourced from Uniprot.org

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