![Western blot analysis of lysates from wild type (WT) and macroH2A. 1 knockout (KO) 293T cells, using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ11100064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89134/357583/STJ11100064_1__90999.1713121716.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and macroH2A. 1 knockout (KO) 293T cells, using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ11100064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and macroH2A. 1 knockout (KO) 293T cells, using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ11100064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89134/357583/STJ11100064_1__90999.1713121716.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and macroH2A. 1 knockout (KO) 293T cells, using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ11100064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
Anti-H2AFY antibody (123-372) (STJ11100064)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal macroH2A.1 (123-372) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MACROH2A1 |
| Gene ID | 9555 |
| Uniprot ID | H2AY_HUMAN |
| Immunogen Region | 123-372 |
| Immunogen Sequence | KLEAIITPPPAKKAKSPSQK KPVSKKAGGKKGARKSKKKQ GEVSKAASADSTTEGTPADG FTVLSTKSLFLGQKLNLIHS EISNLAGFEVEAIINPTNAD IDLKDDLGNTLEKKGGKEFV EAVLELRKKNGPLEVAGAAV SAGHGLPAKFVIHCNSPVWG ADKCEELLEKTVKNCLALAD DKKLKSIAFPSIGSGRNGFP KQTAAQLILKAISSYFVSTM SSSIKTVYFVLFDSESIGI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human macroH2A.1 (NP_613258.2). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin. |
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. Isoform 1: Isoform that specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose and plays a key role in NAD(+) metabolism. Able to bind to the ends of poly-ADP-ribose chains created by PARP1 and cap them. This prevents PARP1 from further addition of ADP-ribose and thus limits the consumption of nuclear NAD(+), allowing the cell to maintain proper NAD(+) levels in both the nucleus and the mitochondria to promote proper mitochondrial respiration. Increases the expression of genes involved in redox metabolism, including SOD3. Isoform 2: In contrast to isoform 1, does not bind poly-ADP-ribose. Represses SOD3 gene expression. |
| Protein Name | Core Histone Macro-H2a.1Histone Macroh2a1Mh2a1Histone H2a.yH2a/YMedulloblastoma Antigen Mu-Mb-50.205 |
| Database Links | |
| Cellular Localisation | NucleusChromosomeEnriched In Inactive X Chromosome Chromatin And In Senescence-Associated HeterochromatinRecruited To Dna Damage Sites In An Aplf-Dependent Manner |
| Alternative Antibody Names | Anti-Core Histone Macro-H2a.1 antibodyAnti-Histone Macroh2a1 antibodyAnti-Mh2a1 antibodyAnti-Histone H2a.y antibodyAnti-H2a/Y antibodyAnti-Medulloblastoma Antigen Mu-Mb-50.205 antibodyAnti-MACROH2A1 antibodyAnti-H2AFY antibody |
Information sourced from Uniprot.org