• Western blot analysis of extracts of various cell lines, using Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) antibody (STJ23899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human stomach cancer using Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) antibody (STJ23899) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) antibody (STJ23899). Blue: DAPI for nuclear staining.

Anti-GYS1 antibody (488-737) (STJ23899)

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STJ23899

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GYS1
Gene ID: 2997
Uniprot ID: GYS1_HUMAN
Immunogen Region: 488-737
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 488-737 of human Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) (NP_002094.2).
Immunogen Sequence: LLPVDYEEFVRGCHLGVFPS YYEPWGYTPAECTVMGIPSI STNLSGFGCFMEEHIADPSA YGIYILDRRFRSLDDSCSQL TSFLYSFCQQSRRQRIIQRN RTERLSDLLDWKYLGRYYMS ARHMALSKAFPEHFTYEPNE ADAAQGYRYPRPASVPPSPS LSRHSSPHQSEDEEDPRNGP LEEDGERYDEDEEAAKDRRN IRAPEWPRRASCTSSTSGSK RNSVDTATSSSLSTPSEPL
Tissue Specificity Expressed in skeletal muscle and most other cell types where glycogen is present.
Post Translational Modifications Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Phosphorylated at Ser-641 by DYRK2, leading to inactivation. Phosphorylated at Ser-641 by PASK, leading to inactivation.phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Function Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Protein Name Glycogen Starch Synthase - Muscle
Glycogen Synthase 1
Database Links Reactome: R-HSA-3322077
Reactome: R-HSA-3785653
Reactome: R-HSA-3814836
Reactome: R-HSA-3828062
Alternative Antibody Names Anti-Glycogen Starch Synthase - Muscle antibody
Anti-Glycogen Synthase 1 antibody
Anti-GYS1 antibody
Anti-GYS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance