• Western blot analysis of extracts of various cell lines, using Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) antibody (STJ23899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human stomach cancer using Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) antibody (STJ23899) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) antibody (STJ23899). Blue: DAPI for nuclear staining.

Anti-GYS1 antibody (488-737) (STJ23899)

SKU:
STJ23899

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GYS1 (488-737) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GYS1
Gene ID: 2997
Uniprot ID: GYS1_HUMAN
Immunogen Region: 488-737
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 488-737 of human Glycogen synthase 1 (Glycogen synthase 1 (GYS1) ) (NP_002094.2).
Immunogen Sequence: LLPVDYEEFVRGCHLGVFPS YYEPWGYTPAECTVMGIPSI STNLSGFGCFMEEHIADPSA YGIYILDRRFRSLDDSCSQL TSFLYSFCQQSRRQRIIQRN RTERLSDLLDWKYLGRYYMS ARHMALSKAFPEHFTYEPNE ADAAQGYRYPRPASVPPSPS LSRHSSPHQSEDEEDPRNGP LEEDGERYDEDEEAAKDRRN IRAPEWPRRASCTSSTSGSK RNSVDTATSSSLSTPSEPL
Post Translational Modifications Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Phosphorylated at Ser-641 by DYRK2, leading to inactivation. Phosphorylated at Ser-641 by PASK, leading to inactivation.phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Function Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Protein Name Glycogen Starch Synthase - Muscle
Database Links Reactome: R-HSA-3322077
Reactome: R-HSA-3785653
Reactome: R-HSA-3814836
Reactome: R-HSA-3828062
Alternative Antibody Names Anti-Glycogen Starch Synthase - Muscle antibody
Anti-GYS1 antibody
Anti-GYS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance