• Western blot analysis of extracts of various cell lines, using GUK1 antibody (STJ111368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using GUK1 antibody (STJ111368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GUK1 antibody (1-197) (STJ111368)

SKU:
STJ111368

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GUK1 (1-197) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GUK1
Gene ID: 2987
Uniprot ID: KGUA_HUMAN
Immunogen Region: 1-197
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human GUK1 (NP_000849.1).
Immunogen Sequence: MSGPRPVVLSGPSGAGKSTL LKRLLQEHSGIFGFSVSHTT RNPRPGEENGKDYYFVTREV MQRDIAAGDFIEHAEFSGNL YGTSKVAVQAVQAMNRICVL DVDLQGVRNIKATDLRPIYI SVQPPSLHVLEQRLRQRNTE TEESLVKRLAAAQADMESSK EPGLFDVVIINDSLDQAYAE LKEALSEEIKKAQRTGA
Tissue Specificity Widely expressed.
Function Catalyzes the phosphorylation of GMP to GDP. Essential enzyme for recycling GMP and indirectly, cyclic GMP (cGMP). Involved in the cGMP metabolism in photoreceptors. It may also have a role in the survival and growth progression of some tumors. In addition to its physiological role, GUK1 is essential for convert prodrugs used for the treatment of cancers and viral infections into their pharmacologically active metabolites, most notably acyclovir, ganciclovir, and 6-thioguanine and its closely related analog 6-mercaptopurine.
Protein Name Guanylate Kinase
Gmp Kinase
Guanylate Kinase 1
Database Links Reactome: R-HSA-499943
Reactome: R-HSA-9748787
Cellular Localisation Photoreceptor Inner Segment
Cytoplasm
Cytosol
Colocalizes With Rd3 In Photoreceptor Inner Segments And To A Lesser Extent In The Outer Plexiform Layer
Alternative Antibody Names Anti-Guanylate Kinase antibody
Anti-Gmp Kinase antibody
Anti-Guanylate Kinase 1 antibody
Anti-GUK1 antibody
Anti-GMK antibody
Anti-GMPK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance