Western blot analysis of lysates from mouse eye, using GUCY2F Rabbit polyclonal antibody (STJ116455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GUCY2F antibody (250-460) (STJ116455)

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STJ116455
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GUCY2F
Gene ID: 2986
Uniprot ID: GUC2F_HUMAN
Immunogen Region: 250-460
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 250-460 of human GUCY2F (NP_001513.2).
Immunogen Sequence: HSALIGGETQMHLLECAHDL KMTDGTYVFVPYDALLYSLP YKHTPYRVLRNNPKLREAYD AVLTITVESQEKTFYQAFTE AAARGEIPEKLEFDQVSPLF GTIYNSIYFIAQAMNNAMKE NGQAGAASLVQHSRNMQFHG FNQLMRTDSNGNGISEYVIL DTNLKEWELHSTYTVDMEME LLRFGGTPIHFPGGRPPRAD AKCWFAEGKIC
Tissue Specificity Retina. Localized exclusively in the outer nuclear layer and inner segments of the rod and cone photoreceptor cells.
Post Translational Modifications There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.
Function Responsible for the synthesis of cyclic GMP (cGMP) in rods and cones of photoreceptors. Plays an essential role in phototransduction, by mediating cGMP replenishment. May also participate in the trafficking of membrane-asociated proteins to the photoreceptor outer segment membrane.
Protein Name Retinal Guanylyl Cyclase 2
Retgc-2
Guanylate Cyclase 2f - Retinal
Guanylate Cyclase F
Gc-F
Rod Outer Segment Membrane Guanylate Cyclase 2
Ros-Gc2
Database Links Reactome: R-HSA-2514859
Cellular Localisation Photoreceptor Outer Segment Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Retinal Guanylyl Cyclase 2 antibody
Anti-Retgc-2 antibody
Anti-Guanylate Cyclase 2f - Retinal antibody
Anti-Guanylate Cyclase F antibody
Anti-Gc-F antibody
Anti-Rod Outer Segment Membrane Guanylate Cyclase 2 antibody
Anti-Ros-Gc2 antibody
Anti-GUCY2F antibody
Anti-GUC2F antibody
Anti-RETGC2 antibody

Information sourced from Uniprot.org

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