• STJ70971 (0. 5µg/ml) staining of Jurkat nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70971 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70971 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and cytoplasmic staining and some plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70971 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-GTF2IRD1 antibody (Internal) (STJ70971)

SKU:
STJ70971-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GTF2IRD1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.5-1µg/ml
IF-Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GTF2IRD1
Gene ID: 9569
Uniprot ID: GT2D1_HUMAN
Immunogen Region: Internal
Accession Number: NP_057412.1; NP_005676.3; NP_001186136.1
Immunogen Sequence: NKFTKDTTKLEPAS
Function May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
Protein Name General Transcription Factor Ii-I Repeat Domain-Containing Protein 1
Gtf2i Repeat Domain-Containing Protein 1
General Transcription Factor Iii
Mustrd1/Ben
Muscle Tfii-I Repeat Domain-Containing Protein 1
Slow-Muscle-Fiber Enhancer-Binding Protein
Use B1-Binding Protein
Williams-Beuren Syndrome Chromosomal Region 11 Protein
Williams-Beuren Syndrome Chromosomal Region 12 Protein
Cellular Localisation Nucleus
Alternative Antibody Names Anti-General Transcription Factor Ii-I Repeat Domain-Containing Protein 1 antibody
Anti-Gtf2i Repeat Domain-Containing Protein 1 antibody
Anti-General Transcription Factor Iii antibody
Anti-Mustrd1/Ben antibody
Anti-Muscle Tfii-I Repeat Domain-Containing Protein 1 antibody
Anti-Slow-Muscle-Fiber Enhancer-Binding Protein antibody
Anti-Use B1-Binding Protein antibody
Anti-Williams-Beuren Syndrome Chromosomal Region 11 Protein antibody
Anti-Williams-Beuren Syndrome Chromosomal Region 12 Protein antibody
Anti-GTF2IRD1 antibody
Anti-CREAM1 antibody
Anti-GTF3 antibody
Anti-MUSTRD1 antibody
Anti-RBAP2 antibody
Anti-WBSCR11 antibody
Anti-WBSCR12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance