• Immunofluorescence analysis of U-2 OS cells using GTF2E1 Rabbit polyclonal antibody (STJ117470) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GTF2E1 Rabbit polyclonal antibody (STJ117470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5S.

Anti-GTF2E1 antibody (230-439) (STJ117470)

SKU:
STJ117470

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTF2E1 (230-439) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF2E1
Gene ID: 2960
Uniprot ID: T2EA_HUMAN
Immunogen Region: 230-439
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-439 of human GTF2E1 (NP_005504.2).
Immunogen Sequence: LAGGHHREAWATKGPSYEDL YTQNVVINMDDQEDLHRASL EGKSAKERPIWLRESTVQGA YGSEDMKEGGIDMDAFQERE EGHAGPDDNEEVMRALLIHE KKTSSAMAGSVGAAAPVTAA NGSDSESETSESDDDSPPRP AAVAVHKREEDEEEDDEFEE VADDPIVMVAGRPFSYSEVS QRPELVAQMTPEEKEAYIAM GQRMFEDLFE
Function Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Protein Name General Transcription Factor Iie Subunit 1
General Transcription Factor Iie 56 Kda Subunit
Transcription Initiation Factor Iie Subunit Alpha
Tfiie-Alpha
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-674695
Reactome: R-HSA-6807505
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Cellular Localisation Nucleus
Alternative Antibody Names Anti-General Transcription Factor Iie Subunit 1 antibody
Anti-General Transcription Factor Iie 56 Kda Subunit antibody
Anti-Transcription Initiation Factor Iie Subunit Alpha antibody
Anti-Tfiie-Alpha antibody
Anti-GTF2E1 antibody
Anti-TF2E1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance