Immunoprecipitation of GST-Tag from 300 Β΅g extracts of 293F cells transfected with a MYOD1 expression vector containing a single N-terminal GST-Tag was performed using 20 Β΅l of Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (STJ11107597). Magnetic Beads-conjugated Rabbit IgG isotype control (STJ11107561) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti GST-Tag mAb (STJ11103710) at a dilution of 1:2000.

Anti-GST-Tag antibody (1-218 aa) [SRM5524] {Magnetic Beads} (STJ11107597)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationMagnetic Beads
IsotypeIgG
STJ11107597
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General Information

Short DescriptionRabbit monoclonal GST-Tag (1-218 aa) antibody for use in IP in species independent samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIP
HostRabbit
ReactivitySpecies Independent
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSRM5524
IsotypeIgG
ConjugationMagnetic Beads
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeIP:20 Mu l-40 Mu l Magnetic Beads for 100 Mu g-300 Mu g extracts of whole cells
FormulationPBS with 0.05% Proclin300, pH 7.3.
Storage InstructionStore at 4Β°C for up to 1 year from the date of receipt. Do not freeze.

Target Information

Immunogen Region1-218 aa
Immunogen SequenceSPILGYWKIKGLVQPTRLLL EYLEEKYEEHLYERDEGDKW RNKKFELGLEFPNLPYYIDG DVKLTQSMAIIRYIADKHNM LGGCPKERAEISMLEGAVLD IRYGVSRIAYSKDFETLKVD FLSKLPEMLKMFEDRLCHKT YLNGDHVTHPDFMLYDALDV VLYMDPMCLDAFPKLVCFKK RIEAIPQIDKYLKSSKYIAW PLQGWQATFGGGDHPPK
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-218 of schja GST protein (P08515).

Additional Info

Background Glutathione S-transferases (GSTs) , previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomalβ€”also known as MAPEGβ€”proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

Information sourced from Uniprot.org

Citations

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