• Western blot analysis of extracts of various cell lines, using Glutathione Synthetase (Glutathione Synthetase (GSS) ) antibody (STJ115456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ115456) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ115456) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ115456) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSS antibody (1-474) (STJ115456)

SKU:
STJ115456

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSS
Gene ID: 2937
Uniprot ID: GSHB_HUMAN
Immunogen Region: 1-474
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-474 of human Glutathione Synthetase (GSS) (NP_000169.1).
Immunogen Sequence: MATNWGSLLQDKQQLEELAR QAVDRALAEGVLLRTSQEPT SSEVVSYAPFTLFPSLVPSA LLEQAYAVQMDFNLLVDAVS QNAAFLEQTLSSTIKQDDFT ARLFDIHKQVLKEGIAQTVF LGLNRSDYMFQRSADGSPAL KQIEINTISASFGGLASRTP AVHRHVLSVLSKTKEAGKIL SNNPSKGLALGIAKAWELYG SPNALVLLIAQEKERNIFDQ RAIENELLARNIHVIRRTF
Function Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes. Participates in ophthalmate biosynthesis in hepatocytes.
Protein Name Glutathione Synthetase
Gsh Synthetase
Gsh-S
Glutathione Synthase
Database Links Reactome: R-HSA-174403
Reactome: R-HSA-5579006
Alternative Antibody Names Anti-Glutathione Synthetase antibody
Anti-Gsh Synthetase antibody
Anti-Gsh-S antibody
Anti-Glutathione Synthase antibody
Anti-GSS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance