| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DFNA5/GSDME (271-496) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GSDME |
| Gene ID: |
1687 |
| Uniprot ID: |
GSDME_HUMAN |
| Immunogen Region: |
271-496 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 271-496 of human GSDME (NP_004394.1). |
| Immunogen Sequence: |
AAHGISSQDGPLSVLKQATL LLERNFHPFAELPEPQQTAL SDIFQAVLFDDELLMVLEPV CDDLVSGLSPTVAVLGELKP RQQQDLVAFLQLVGCSLQGG CPGPEDAGSKQLFMTAYFLV SALAEMPDSAAALLGTCCKL QIIPTLCHLLRALSDDGVSD LEDPTLTPLKDTERFGIVQR LFASADISLERLKSSVKAVI LKDSKVFPLLLCITLNGLCA LGREHS |
| Tissue Specificity | Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta. |
| Post Translational Modifications | Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) or granzyme B (GZMB) relieves autoinhibition and is sufficient to initiate pyroptosis. Gasdermin-E: Succination by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits processing by caspases, and ability to initiate pyroptosis. Succination modification is catalyzed by a non-enzymatic reaction caused by an accumulation of fumarate. |
| Function | Gasdermin-E: Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis. This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis. Gasdermin-E, N-terminal: Pore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively. After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukins (IL1B and IL16) and triggering pyroptosis. Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents. Exhibits bactericidal activity. Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity. Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells: cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression. May play a role in the p53/TP53-regulated cellular response to DNA damage. Gasdermin-E, N-terminal: (Microbial infection) Pore-forming protein, which promotes maternal placental pyroptosis in response to Zika virus infection, contributing to adverse fetal outcomes. |
| Protein Name | Gasdermin-EInversely Correlated With Estrogen Receptor Expression 1Icere-1Non-Syndromic Hearing Impairment Protein 5 Cleaved Into - Gasdermin-E - N-TerminalGsdme-Nt - Gasdermin-E - C-TerminalGsdme-Ct |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-5620971Reactome: R-HSA-5686938Reactome: R-HSA-9710421 |
| Cellular Localisation | Gasdermin-EN-Terminal: Cell MembraneMulti-Pass Membrane ProteinGasdermin-E: CytoplasmCytosol |
| Alternative Antibody Names | Anti-Gasdermin-E antibodyAnti-Inversely Correlated With Estrogen Receptor Expression 1 antibodyAnti-Icere-1 antibodyAnti-Non-Syndromic Hearing Impairment Protein 5 Cleaved Into - Gasdermin-E - N-Terminal antibodyAnti-Gsdme-Nt - Gasdermin-E - C-Terminal antibodyAnti-Gsdme-Ct antibodyAnti-GSDME antibodyAnti-DFNA5 antibodyAnti-ICERE1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
