| Host: |
Mouse |
| Applications: |
WB/ICC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Grb2 (Full Length) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M331 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000ICC 1:50IP 1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Grb2 |
| Gene ID: |
81504 |
| Uniprot ID: |
GRB2_RAT |
| Immunogen Region: |
Full Length |
| Specificity: |
This antibody detects a 24 kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12. |
| Immunogen: |
Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2. |
| Tissue Specificity | Wide tissue distribution. |
| Post Translational Modifications | Phosphorylation of Tyr-209 in the C-terminal SH3 domain reduces its binding to SOS1. Ubiquitinated by RNF173, leading to proteasomal degradation and inhibition of the RAF/MEK/ERK pathway. In the nucleus, polyubiquitinated by RBBP6 at Lys-109 at DNA damage sites. |
| Function | Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis. Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells. Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2. In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation. Functions also a role in B-cell activation by amplifying Ca(2+) mobilization and activation of the ERK MAP kinase pathway upon recruitment to the phosphorylated B-cell antigen receptor (BCR). Plays a role in switching between autophagy and programmed necrosis upstream of EGFR by interacting with components of necrosomes including RIPK1 and with autophagy regulators SQSTM1 and BECN1. Regulates miRNA biogenesis by forming a functional ternary complex with AGO2 and DICER1. Functions in the replication stress response by protecting DNA at stalled replication forks from MRE11-mediated degradation. Mechanistically, inhibits RAD51 ATPase activity to stabilize RAD51 on stalled replication forks. Additionally, directly recruits and later releases MRE11 at DNA damage sites during the homology-directed repair (HDR) process. |
| Protein Name | Growth Factor Receptor-Bound Protein 2Adapter Protein Grb2Protein AshSh2/Sh3 Adapter Grb2 |
| Database Links | Reactome: R-RNO-109704Reactome: -RNO-112412Reactome: -RNO-1250347Reactome: -RNO-1257604Reactome: -RNO-1295596Reactome: -RNO-1433557Reactome: -RNO-1433559Reactome: -RNO-167044Reactome: -RNO-179812Reactome: -RNO-180292Reactome: -RNO-180336Reactome: -RNO-182971Reactome: -RNO-186763Reactome: -RNO-1963640Reactome: -RNO-1963642Reactome: -RNO-2029482Reactome: -RNO-210993Reactome: -RNO-2179392Reactome: -RNO-2424491Reactome: -RNO-2428933Reactome: -RNO-2730905Reactome: -RNO-2871796Reactome: -RNO-2871809Reactome: -RNO-354194Reactome: -RNO-375165Reactome: -RNO-388841Reactome: -RNO-389359Reactome: -RNO-391160Reactome: -RNO-5654688Reactome: -RNO-5654689Reactome: -RNO-5654693Reactome: -RNO-5654695Reactome: -RNO-5654699Reactome: -RNO-5654700Reactome: -RNO-5654704Reactome: -RNO-5654706Reactome: -RNO-5654710Reactome: -RNO-5654712Reactome: -RNO-5654719Reactome: -RNO-5654720Reactome: -RNO-5654726Reactome: -RNO-5654727Reactome: -RNO-5654732Reactome: -RNO-5654733Reactome: -RNO-5663213Reactome: -RNO-5673001Reactome: -RNO-6807004Reactome: -RNO-6811558Reactome: -RNO-74749Reactome: -RNO-74751Reactome: -RNO-8851805Reactome: -RNO-8851907Reactome: -RNO-8853659Reactome: -RNO-8856825Reactome: -RNO-8856828Reactome: -RNO-8865999Reactome: -RNO-8875555Reactome: -RNO-8875656Reactome: -RNO-8983432Reactome: -RNO-9013420Reactome: -RNO-9027284Reactome: -RNO-9028731Reactome: -RNO-912526Reactome: -RNO-912631Reactome: -RNO-9607240Reactome: -RNO-9674555Reactome: -RNO-983695Reactome: -RNO-9842663 |
| Cellular Localisation | NucleusCytoplasmEndosomeGolgi Apparatus |
| Alternative Antibody Names | Anti-Growth Factor Receptor-Bound Protein 2 antibodyAnti-Adapter Protein Grb2 antibodyAnti-Protein Ash antibodyAnti-Sh2/Sh3 Adapter Grb2 antibodyAnti-Grb2 antibodyAnti-Ash antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
