• Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g GRB2 antibody (STJ11101597). Western blot was performed from the immunoprecipitate using GRB2 (STJ11101597) at a dilution of 1:1000.
  • Confocal imaging of HeLa cells using GRB2 Rabbit monoclonal antibody (STJ11101597, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of GRB2 in paraffin-embedded rat spleen using GRB2 Rabbit monoclonal antibody (STJ11101597) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GRB2 in paraffin-embedded human tonsil using GRB2 Rabbit monoclonal antibody (STJ11101597) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GRB2 in paraffin-embedded human breast cancer using GRB2 Rabbit monoclonal antibody (STJ11101597) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GRB2 Rabbit monoclonal antibody (STJ11101597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GRB2 antibody (118-217) [S7MR] (STJ11101597)

SKU:
STJ11101597

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GRB2 (118-217) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRB2
Gene ID: 2885
Uniprot ID: GRB2_HUMAN
Immunogen Region: 118-217
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 118-217 of human GRB2 (P62993).
Immunogen Sequence: YFLWVVKFNSLNELVDYHRS TSVSRNQQIFLRDIEQVPQQ PTYVQALFDFDPQEDGELGF RRGDFIHVMDNSDPNWWKGA CHGQTGMFPRNYVTPVNRNV
Function Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform 2: Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Protein Name Growth Factor Receptor-Bound Protein 2
Adapter Protein Grb2
Protein Ash
Sh2/Sh3 Adapter Grb2
Database Links Reactome: R-HSA-109704 P62993-1
Reactome: R-HSA-112412 P62993-1
Reactome: R-HSA-1236382 P62993-1
Reactome: R-HSA-1250196 P62993-1
Reactome: R-HSA-1250347 P62993-1
Reactome: R-HSA-1257604 P62993-1
Reactome: R-HSA-1295596 P62993-1
Reactome: R-HSA-1433557 P62993-1
Reactome: R-HSA-1433559 P62993-1
Reactome: R-HSA-167044 P62993-1
Reactome: R-HSA-179812 P62993-1
Reactome: R-HSA-180292 P62993-1
Reactome: R-HSA-180336 P62993-1
Reactome: R-HSA-182971 P62993-1
Reactome: R-HSA-1839117 P62993-1
Reactome: R-HSA-186763 P62993-1
Reactome: R-HSA-1963640 P62993-1
Reactome: R-HSA-1963642 P62993-1
Reactome: R-HSA-2029482 P62993-1
Reactome: R-HSA-210993 P62993-1
Reactome: R-HSA-2179392 P62993-1
Reactome: R-HSA-2219530 P62993-1
Reactome: R-HSA-2424491 P62993-1
Reactome: R-HSA-2428933 P62993-1
Reactome: R-HSA-2730905 P62993-1
Reactome: R-HSA-2871796 P62993-1
Reactome: R-HSA-2871809 P62993-1
Reactome: R-HSA-354194 P62993-1
Reactome: R-HSA-375165 P62993-1
Reactome: R-HSA-388841 P62993-1
Reactome: R-HSA-389359 P62993-1
Reactome: R-HSA-391160 P62993-1
Reactome: R-HSA-512988 P62993-1
Reactome: R-HSA-5637810 P62993-1
Reactome: R-HSA-5654688 P62993-1
Reactome: R-HSA-5654689 P62993-1
Reactome: R-HSA-5654693 P62993-1
Reactome: R-HSA-5654695 P62993-1
Reactome: R-HSA-5654699 P62993-1
Reactome: R-HSA-5654700 P62993-1
Reactome: R-HSA-5654704 P62993-1
Reactome: R-HSA-5654706 P62993-1
Reactome: R-HSA-5654710 P62993-1
Reactome: R-HSA-5654712 P62993-1
Reactome: R-HSA-5654719 P62993-1
Reactome: R-HSA-5654720 P62993-1
Reactome: R-HSA-5654726 P62993-1
Reactome: R-HSA-5654727 P62993-1
Reactome: R-HSA-5654732 P62993-1
Reactome: R-HSA-5654733 P62993-1
Reactome: R-HSA-5655253 P62993-1
Reactome: R-HSA-5655291 P62993-1
Reactome: R-HSA-5655302 P62993-1
Reactome: R-HSA-5655332 P62993-1
Reactome: R-HSA-5663213 P62993-1
Reactome: R-HSA-5673001 P62993-1
Reactome: R-HSA-6807004 P62993-1
Reactome: R-HSA-6811558 P62993-1
Reactome: R-HSA-74749 P62993-1
Reactome: R-HSA-74751 P62993-1
Reactome: R-HSA-8851805 P62993-1
Reactome: R-HSA-8851907 P62993-1
Reactome: R-HSA-8853659 P62993-1
Reactome: R-HSA-8856825 P62993-1
Reactome: R-HSA-8856828 P62993-1
Reactome: R-HSA-8865999 P62993-1
Reactome: R-HSA-8875360 P62993-1
Reactome: R-HSA-8875555 P62993-1
Reactome: R-HSA-8875656 P62993-1
Reactome: R-HSA-8983432
Reactome: R-HSA-9013420 P62993-1
Reactome: R-HSA-9026519 P62993-1
Reactome: R-HSA-9027284 P62993-1
Reactome: R-HSA-9028335 P62993-1
Reactome: R-HSA-9028731 P62993-1
Reactome: R-HSA-9034864 P62993-1
Reactome: R-HSA-912526 P62993-1
Reactome: R-HSA-912631 P62993-1
Reactome: R-HSA-9607240 P62993-1
Reactome: R-HSA-9634285 P62993-1
Reactome: R-HSA-9645135 P62993-1
Reactome: R-HSA-9664422 P62993-1
Reactome: R-HSA-9664565 P62993-1
Reactome: R-HSA-9665348 P62993-1
Reactome: R-HSA-9665686 P62993-1
Reactome: R-HSA-9670439 P62993-1
Reactome: R-HSA-9673767 P62993-1
Reactome: R-HSA-9673770 P62993-1
Reactome: R-HSA-9674555
Reactome: R-HSA-9679191 P62993-1
Reactome: R-HSA-9680350 P62993-1
Reactome: R-HSA-9702518
Reactome: R-HSA-9703465 P62993-1
Reactome: R-HSA-9703648
Reactome: R-HSA-9725370
Reactome: R-HSA-983695 P62993-1
Cellular Localisation Nucleus
Cytoplasm
Endosome
Golgi Apparatus
Alternative Antibody Names Anti-Growth Factor Receptor-Bound Protein 2 antibody
Anti-Adapter Protein Grb2 antibody
Anti-Protein Ash antibody
Anti-Sh2/Sh3 Adapter Grb2 antibody
Anti-GRB2 antibody
Anti-ASH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance