• Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g GRB2 antibody (STJ11101597). Western blot was performed from the immunoprecipitate using GRB2 (STJ11101597) at a dilution of 1:1000.
  • Confocal imaging of HeLa cells using GRB2 Rabbit monoclonal antibody (STJ11101597, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of GRB2 in paraffin-embedded rat spleen using GRB2 Rabbit monoclonal antibody (STJ11101597) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GRB2 in paraffin-embedded human tonsil using GRB2 Rabbit monoclonal antibody (STJ11101597) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GRB2 in paraffin-embedded human breast cancer using GRB2 Rabbit monoclonal antibody (STJ11101597) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GRB2 Rabbit monoclonal antibody (STJ11101597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GRB2 antibody (118-217) [S7MR] (STJ11101597)

SKU:
STJ11101597

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:200-1:800
IF/ICC:1:100-1:800
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your spe
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRB2
Gene ID: 2885
Uniprot ID: GRB2_HUMAN
Immunogen Region: 118-217
Specificity: A synthetic peptide corresponding to a sequence within amino acids 118-217 of human GRB2 (P62993).
Immunogen Sequence: YFLWVVKFNSLNELVDYHRS TSVSRNQQIFLRDIEQVPQQ PTYVQALFDFDPQEDGELGF RRGDFIHVMDNSDPNWWKGA CHGQTGMFPRNYVTPVNRNV
Post Translational Modifications Phosphorylation of Tyr-209 in the C-terminal SH3 domain reduces its binding to SOS1. Ubiquitinated by RNF173, leading to proteasomal degradation and inhibition of the RAF/MEK/ERK pathway. In the nucleus, polyubiquitinated by RBBP6 at Lys-109 at DNA damage sites.
Function Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis. Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells. Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2. In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation. Functions also a role in B-cell activation by amplifying Ca(2+) mobilization and activation of the ERK MAP kinase pathway upon recruitment to the phosphorylated B-cell antigen receptor (BCR). Plays a role in switching between autophagy and programmed necrosis upstream of EGFR by interacting with components of necrosomes including RIPK1 and with autophagy regulators SQSTM1 and BECN1. Regulates miRNA biogenesis by forming a functional ternary complex with AGO2 and DICER1. Functions in the replication stress response by protecting DNA at stalled replication forks from MRE11-mediated degradation. Mechanistically, inhibits RAD51 ATPase activity to stabilize RAD51 on stalled replication forks. Additionally, directly recruits and later releases MRE11 at DNA damage sites during the homology-directed repair (HDR) process. Isoform 2: Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. Mechanistically, inhibits RAS-ERK signaling and downstream cell proliferation by competing with GRB2 for SOS1 binding and thus by regulating SOS1 membrane recruitment.
Protein Name Growth Factor Receptor-Bound Protein 2
Adapter Protein Grb2
Protein Ash
Sh2/Sh3 Adapter Grb2
Database Links Reactome: R-HSA-109704 P62993-1
Reactome: R-HSA-112412 P62993-1
Reactome: R-HSA-1236382 P62993-1
Reactome: R-HSA-1250196 P62993-1
Reactome: R-HSA-1250347 P62993-1
Reactome: R-HSA-1257604 P62993-1
Reactome: R-HSA-1295596 P62993-1
Reactome: R-HSA-1433557 P62993-1
Reactome: R-HSA-1433559 P62993-1
Reactome: R-HSA-167044 P62993-1
Reactome: R-HSA-179812 P62993-1
Reactome: R-HSA-180292 P62993-1
Reactome: R-HSA-180336 P62993-1
Reactome: R-HSA-182971 P62993-1
Reactome: R-HSA-1839117 P62993-1
Reactome: R-HSA-186763 P62993-1
Reactome: R-HSA-1963640 P62993-1
Reactome: R-HSA-1963642 P62993-1
Reactome: R-HSA-2029482 P62993-1
Reactome: R-HSA-210993 P62993-1
Reactome: R-HSA-2179392 P62993-1
Reactome: R-HSA-2219530 P62993-1
Reactome: R-HSA-2424491 P62993-1
Reactome: R-HSA-2428933 P62993-1
Reactome: R-HSA-2730905 P62993-1
Reactome: R-HSA-2871796 P62993-1
Reactome: R-HSA-2871809 P62993-1
Reactome: R-HSA-354194 P62993-1
Reactome: R-HSA-375165 P62993-1
Reactome: R-HSA-388841 P62993-1
Reactome: R-HSA-389359 P62993-1
Reactome: R-HSA-391160 P62993-1
Reactome: R-HSA-512988 P62993-1
Reactome: R-HSA-5637810 P62993-1
Reactome: R-HSA-5654688 P62993-1
Reactome: R-HSA-5654689 P62993-1
Reactome: R-HSA-5654693 P62993-1
Reactome: R-HSA-5654695 P62993-1
Reactome: R-HSA-5654699 P62993-1
Reactome: R-HSA-5654700 P62993-1
Reactome: R-HSA-5654704 P62993-1
Reactome: R-HSA-5654706 P62993-1
Reactome: R-HSA-5654710 P62993-1
Reactome: R-HSA-5654712 P62993-1
Reactome: R-HSA-5654719 P62993-1
Reactome: R-HSA-5654720 P62993-1
Reactome: R-HSA-5654726 P62993-1
Reactome: R-HSA-5654727 P62993-1
Reactome: R-HSA-5654732 P62993-1
Reactome: R-HSA-5654733 P62993-1
Reactome: R-HSA-5655253 P62993-1
Reactome: R-HSA-5655291 P62993-1
Reactome: R-HSA-5655302 P62993-1
Reactome: R-HSA-5655332 P62993-1
Reactome: R-HSA-5663213 P62993-1
Reactome: R-HSA-5673001 P62993-1
Reactome: R-HSA-6807004 P62993-1
Reactome: R-HSA-6811558 P62993-1
Reactome: R-HSA-74749 P62993-1
Reactome: R-HSA-74751 P62993-1
Reactome: R-HSA-8851805 P62993-1
Reactome: R-HSA-8851907 P62993-1
Reactome: R-HSA-8853659 P62993-1
Reactome: R-HSA-8856825 P62993-1
Reactome: R-HSA-8856828 P62993-1
Reactome: R-HSA-8865999 P62993-1
Reactome: R-HSA-8875360 P62993-1
Reactome: R-HSA-8875555 P62993-1
Reactome: R-HSA-8875656 P62993-1
Reactome: R-HSA-8983432
Reactome: R-HSA-9013420 P62993-1
Reactome: R-HSA-9026519 P62993-1
Reactome: R-HSA-9027284 P62993-1
Reactome: R-HSA-9028335 P62993-1
Reactome: R-HSA-9028731 P62993-1
Reactome: R-HSA-9034864 P62993-1
Reactome: R-HSA-912526 P62993-1
Reactome: R-HSA-912631 P62993-1
Reactome: R-HSA-9607240 P62993-1
Reactome: R-HSA-9634285 P62993-1
Reactome: R-HSA-9645135 P62993-1
Reactome: R-HSA-9664422 P62993-1
Reactome: R-HSA-9664565 P62993-1
Reactome: R-HSA-9665348 P62993-1
Reactome: R-HSA-9665686 P62993-1
Reactome: R-HSA-9670439 P62993-1
Reactome: R-HSA-9673767 P62993-1
Reactome: R-HSA-9673770 P62993-1
Reactome: R-HSA-9674555
Reactome: R-HSA-9679191 P62993-1
Reactome: R-HSA-9680350 P62993-1
Reactome: R-HSA-9702518
Reactome: R-HSA-9703465 P62993-1
Reactome: R-HSA-9703648
Reactome: R-HSA-9725370
Reactome: R-HSA-983695 P62993-1
Reactome: R-HSA-9842663
Cellular Localisation Nucleus
Cytoplasm
Endosome
Golgi Apparatus
Alternative Antibody Names Anti-Growth Factor Receptor-Bound Protein 2 antibody
Anti-Adapter Protein Grb2 antibody
Anti-Protein Ash antibody
Anti-Sh2/Sh3 Adapter Grb2 antibody
Anti-GRB2 antibody
Anti-ASH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance