Anti-Granulin/GRN antibody (Internal) (STJ71736)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Granulin/GRN (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
| Applications | Pep-ELISA/IF |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments showed a band at approx 65kDa in Human Bone Marrow and Testes lysates after 2µg/ml antibody staining (calculated MW of 63.5kDa according to NP_002078.1). Primary |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | GRN |
| Gene ID | 2896 |
| Uniprot ID | GRN_HUMAN |
| Accession Number | NP_002078.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | QSKCLSKENATTD |
Additional Info
| Post Translational Modifications | Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome. |
| Function | Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-4: Promotes proliferation of the epithelial cell line A431 in culture. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. |
| Protein Name | ProgranulinPgrnAcrograninEpithelin PrecursorGlycoprotein Of 88 KdaGp88Glycoprotein 88Granulin PrecursorPc Cell-Derived Growth FactorPcdgfProepithelinPepi Cleaved Into - Paragranulin - Granulin-1Granulin G - Granulin-2Granulin F - Granulin-3Epithelin-2Granulin B - Granulin-4Epithelin-1Granulin A - Granulin-5Granulin C - Granulin-6Granulin D - Granulin-7Granulin E |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedLysosomeEndocytosed By Sort1 And Delivred To LysosomesTargeted To Lysosome By Psap Via M6pr And Lrp1In Both Biosynthetic And Endocytic PathwaysCo-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome |
| Alternative Antibody Names | Anti-Progranulin antibodyAnti-Pgrn antibodyAnti-Acrogranin antibodyAnti-Epithelin Precursor antibodyAnti-Glycoprotein Of 88 Kda antibodyAnti-Gp88 antibodyAnti-Glycoprotein 88 antibodyAnti-Granulin Precursor antibodyAnti-Pc Cell-Derived Growth Factor antibodyAnti-Pcdgf antibodyAnti-Proepithelin antibodyAnti-Pepi Cleaved Into - Paragranulin - Granulin-1 antibodyAnti-Granulin G - Granulin-2 antibodyAnti-Granulin F - Granulin-3 antibodyAnti-Epithelin-2 antibodyAnti-Granulin B - Granulin-4 antibodyAnti-Epithelin-1 antibodyAnti-Granulin A - Granulin-5 antibodyAnti-Granulin C - Granulin-6 antibodyAnti-Granulin D - Granulin-7 antibodyAnti-Granulin E antibodyAnti-GRN antibody |
Information sourced from Uniprot.org