| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-GRAF/ARHGAP26 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 90kDa band observed in Human Heart lysates (calculated MW of 92.2kDa according to NP_055886.1). Recommended concentration: 0.3-1µg/ml. An additional strong band of unknown ident |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ARHGAP26 |
| Gene ID: | 23092 |
| Uniprot ID: | RHG26_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_055886.1; NP_001129080.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms. |
| Immunogen Sequence: | KTGLIPENYVEFL |
| Function | GTPase-activating protein for RHOA and CDC42. Facilitates mitochondrial quality control by promoting Parkin-mediated recruitment of autophagosomes to damaged mitochondria. Negatively regulates the growth of human parainfluenza virus type 2 by inhibiting hPIV-2-mediated RHOA activation via interaction with two of its viral proteins P and V. Isoform 2: Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation of MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins. |
| Protein Name | Rho Gtpase-Activating Protein 26Gtpase Regulator Associated With Focal Adhesion KinaseGraf1Oligophrenin-1-Like ProteinRho-Type Gtpase-Activating Protein 26 |
| Database Links | Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9013423 |
| Cellular Localisation | Isoform 2: Endosome MembraneColocalized With Rab8aRab8b And Rab10 On Endosomal TubulesCytoplasmCell JunctionFocal AdhesionCytoskeletonColocalizes With Actin Stress Fibers And Cortical Actin Structures |
| Alternative Antibody Names | Anti-Rho Gtpase-Activating Protein 26 antibodyAnti-Gtpase Regulator Associated With Focal Adhesion Kinase antibodyAnti-Graf1 antibodyAnti-Oligophrenin-1-Like Protein antibodyAnti-Rho-Type Gtpase-Activating Protein 26 antibodyAnti-ARHGAP26 antibodyAnti-GRAF antibodyAnti-KIAA0621 antibodyAnti-OPHN1L antibody |
Information sourced from Uniprot.org
