• Immunofluorescence analysis of 293T cells using GPX1 Rabbit polyclonal antibody (STJ23851) at dilution of 1:200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using GPX1 Rabbit polyclonal antibody (STJ23851) at dilution of 1:200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GPX1 Rabbit polyclonal antibody (STJ23851) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : MCF7. Exposuretime: 60s.

Anti-GPX1 antibody (100-200) (STJ23851)

SKU:
STJ23851

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPX1
Gene ID: 2876
Uniprot ID: GPX1_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GPX1 (NP_000572.2).
Immunogen Sequence: RPGGGFEPNFMLFEKCEVNG AGAHPLFAFLREALPAPSDD ATALMTDPKLITWSPVCRND VAWNFEKFLVGPDGVPLRRY SRRFQTIDIEPDIEALLSQG P
Tissue Specificity Expressed in platelets (at protein level).
Post Translational Modifications During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
Function Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway.
Protein Name Glutathione Peroxidase 1
Gpx-1
Gshpx-1
Cellular Glutathione Peroxidase
Phospholipid-Hydroperoxide Glutathione Peroxidase Gpx1
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-2142712
Reactome: R-HSA-2142770
Reactome: R-HSA-3299685
Cellular Localisation Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-Glutathione Peroxidase 1 antibody
Anti-Gpx-1 antibody
Anti-Gshpx-1 antibody
Anti-Cellular Glutathione Peroxidase antibody
Anti-Phospholipid-Hydroperoxide Glutathione Peroxidase Gpx1 antibody
Anti-GPX1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance