• Western blot analysis of extracts of A-431 cells, using GPRC5D antibody (STJ119764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPRC5D antibody (264-345) (STJ119764)

SKU:
STJ119764

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPRC5D
Gene ID: 55507
Uniprot ID: GPC5D_HUMAN
Immunogen Region: 264-345
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 264-345 of human GPRC5D (NP_061124.1).
Immunogen Sequence: ILYRSCRQECPLQGNACPVT AYQHSFQVENQELSRARDSD GAEEDVALTSYGTPIQPQTV DPTQECFIPQAKLSPQQDAG GV
Tissue Specificity Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
Protein Name G-Protein Coupled Receptor Family C Group 5 Member D
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-G-Protein Coupled Receptor Family C Group 5 Member D antibody
Anti-GPRC5D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance