• STJ70946 (0. 1µg/ml) staining of U2OS (A) , HEK293 (B) , HeLa (C) and (0. 3ug/ml) A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminecence.
  • STJ70946 (0. 1µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70946 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70946 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70946 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-GPR94/TRA1 antibody (Internal) (STJ70946)

SKU:
STJ70946-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GPR94/TRA1 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:8000.
WB: Approx. 100kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS and in preliminary testing of NIH3T3 cell lysate and Mouse Liver lysate (calculated MW of 92.5kDa according to
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HSP90B1
Gene ID: 7184
Uniprot ID: ENPL_HUMAN
Immunogen Region: Internal
Accession Number: NP_003290.1
Immunogen Sequence: KEGVKFDESEKTKE
Protein Name Endoplasmin
94 Kda Glucose-Regulated Protein
Grp-94
Heat Shock Protein 90 Kda Beta Member 1
Heat Shock Protein Family C Member 4
Tumor Rejection Antigen 1
Gp96 Homolog
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-3000480
Reactome: R-HSA-381183
Reactome: R-HSA-381426
Reactome: R-HSA-6785807
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Endoplasmin antibody
Anti-94 Kda Glucose-Regulated Protein antibody
Anti-Grp-94 antibody
Anti-Heat Shock Protein 90 Kda Beta Member 1 antibody
Anti-Heat Shock Protein Family C Member 4 antibody
Anti-Tumor Rejection Antigen 1 antibody
Anti-Gp96 Homolog antibody
Anti-HSP90B1 antibody
Anti-GRP94 antibody
Anti-HSPC4 antibody
Anti-TRA1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance