• STJ70946 (0. 1µg/ml) staining of U2OS (A) , HEK293 (B) , HeLa (C) and (0. 3ug/ml) A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminecence.
  • STJ70946 (0. 1µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70946 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70946 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70946 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-GPR94/TRA1 antibody (Internal) (STJ70946)

SKU:
STJ70946-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GPR94/TRA1 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3-5µg/ml
IF-Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HSP90B1
Gene ID: 7184
Uniprot ID: ENPL_HUMAN
Immunogen Region: Internal
Accession Number: NP_003290.1
Immunogen Sequence: KEGVKFDESEKTKE
Protein Name Endoplasmin
94 Kda Glucose-Regulated Protein
Grp-94
Heat Shock Protein 90 Kda Beta Member 1
Tumor Rejection Antigen 1
Gp96 Homolog
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-3000480
Reactome: R-HSA-381183
Reactome: R-HSA-381426
Reactome: R-HSA-6785807
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Endoplasmin antibody
Anti-94 Kda Glucose-Regulated Protein antibody
Anti-Grp-94 antibody
Anti-Heat Shock Protein 90 Kda Beta Member 1 antibody
Anti-Tumor Rejection Antigen 1 antibody
Anti-Gp96 Homolog antibody
Anti-HSP90B1 antibody
Anti-GRP94 antibody
Anti-TRA1 antibody

Information sourced from Uniprot.org

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