Anti-GPR68 antibody (281-330 aa) (STJ93394)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human SPR1 at the amino acid range 281-330
General Information
| Short Description | Rabbit polyclonal anti-Ovarian cancer G-protein coupled receptor 1 (281-330 aa) for use in WB, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPR68 |
| Gene ID | 8111 |
| Uniprot ID | GPR68_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human SPR1 at the amino acid range 281-330 |
| Immunogen Region | 281-330 aa |
| Specificity | GPR68 Polyclonal Antibody detects endogenous levels of GPR68 protein. |
Additional Info
| Function | Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as phospholipase C. GPR68 is mainly coupled to G(q) G proteins and mediates production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Acts as a key mechanosensor of fluid shear stress and membrane stretch. Expressed in endothelial cells of small-diameter resistance arteries, where it mediates flow-induced dilation in response to shear stress. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Acts as a regulator of calcium-sensing receptor CASR in a seesaw manner: GPR68-mediated signaling inhibits CASR signaling in response to protons, while CASR inhibits GPR68 in presence of extracellular calcium. |
| Protein Name | G-Protein Coupled Receptor 68G-Protein Coupled Receptor 12aGpr12aOvarian Cancer G-Protein Coupled Receptor 1Ogr-1 |
| Database Links | Reactome: R-HSA-373076Reactome: R-HSA-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-G-Protein Coupled Receptor 68 antibodyAnti-G-Protein Coupled Receptor 12a antibodyAnti-Gpr12a antibodyAnti-Ovarian Cancer G-Protein Coupled Receptor 1 antibodyAnti-Ogr-1 antibodyAnti-GPR68 antibodyAnti-OGR1 antibody |
Information sourced from Uniprot.org