Anti-GPR35 antibody (51-100 aa) (STJ93380)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GPR35 at the amino acid range 51-100
General Information
| Short Description | Rabbit polyclonal anti-G-protein coupled receptor 35 (51-100 aa) for use in WB, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPR35 |
| Gene ID | 2859 |
| Uniprot ID | GPR35_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GPR35 at the amino acid range 51-100 |
| Immunogen Region | 51-100 aa |
| Specificity | GPR35 Polyclonal Antibody detects endogenous levels of GPR35 protein. |
Additional Info
| Post Translational Modifications | Multiply phosphorylated in clusters of serines and threonines in the C-terminal tail. Phosphorylation of Ser-300 and Ser-303 is mediated by GRK5 and/or GRK6. |
| Function | G-protein coupled receptor that binds to several ligands including the tryptophan metabolite kynurenic acid (KYNA), lysophosphatidic acid (LPA) or 5-hydroxyindoleacetic acid (5-HIAA) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Plays a role in neutrophil recruitment to sites of inflammation and bacterial clearance through the major serotonin metabolite 5-HIAA that acts as a physiological ligand. Stimulates lipid metabolism, thermogenic, and anti-inflammatory gene expression in adipose tissue once activated by kynurenic acid. In macrophages, activation by lysophosphatidic acid promotes GPR35-induced signaling with a distinct transcriptional profile characterized by TNF production associated with ERK and NF-kappa-B activation. In turn, induces chemotaxis of macrophages. |
| Protein Name | G-Protein Coupled Receptor 35Kynurenic Acid ReceptorKyna Receptor |
| Database Links | Reactome: R-HSA-373076 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinInternalized To The Cytoplasm After Exposure To Kynurenic Acid |
| Alternative Antibody Names | Anti-G-Protein Coupled Receptor 35 antibodyAnti-Kynurenic Acid Receptor antibodyAnti-Kyna Receptor antibodyAnti-GPR35 antibody |
Information sourced from Uniprot.org