Anti-GPC3 antibody (400-500 aa) [ABT068] (STJ197234)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenSynthesized peptide derived from the human Glypican-3 (GPC3) at the amino acid range 400-500
General Information
| Short Description | Mouse monoclonal anti-Glypican-3 (400-500 aa) for use in IHC, WB and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB/IF |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT068 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:100-500WB 1:200-1000IF 1:100-500 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPC3 |
| Gene ID | 2719 |
| Uniprot ID | GPC3_HUMAN |
| Immunogen | Synthesized peptide derived from the human Glypican-3 (GPC3) at the amino acid range 400-500 |
| Immunogen Region | 400-500 aa |
| Specificity | The antibody can specifically recognize human Glypican-3 protein. |
Additional Info
| Post Translational Modifications | O-glycosylated.contains heparan sulfate and/or chondroitin sulfate. Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor. This processing is essential for its role in inhibition of hedgehog signaling. A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface. |
| Function | Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation. |
| Protein Name | Glypican-3Gtr2-2Intestinal Protein Oci-5Mxr7 Cleaved Into - Glypican-3 Alpha Subunit - Glypican-3 Beta Subunit |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022928Reactome: R-HSA-2024096Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3656237Reactome: R-HSA-3656253Reactome: R-HSA-381426Reactome: R-HSA-4420332Reactome: R-HSA-8957275Reactome: R-HSA-9694614Reactome: R-HSA-975634Reactome: R-HSA-9820960Reactome: R-HSA-9833110 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorExtracellular Side |
| Alternative Antibody Names | Anti-Glypican-3 antibodyAnti-Gtr2-2 antibodyAnti-Intestinal Protein Oci-5 antibodyAnti-Mxr7 Cleaved Into - Glypican-3 Alpha Subunit - Glypican-3 Beta Subunit antibodyAnti-GPC3 antibodyAnti-OCI5 antibody |
Information sourced from Uniprot.org