• Western blot analysis of various lysates using (STJ28693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPAM antibody (594-828) (STJ28693)

SKU:
STJ28693

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPAM
Gene ID: 57678
Uniprot ID: GPAT1_HUMAN
Immunogen Region: 594-828
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_001231878.1).
Immunogen Sequence: LNKRGLGGPTSTPPNLISQE QLVRKAASLCYLLSNEGTIS LPCQTFYQVCHETVGKFIQY GILTVAEHDDQEDISPSLAE QQWDKKLPEPLSWRSDEEDE DSDFGEEQRDCYLKVSQSKE HQQFITFLQRLLGPLLEAYS SAAIFVHNFSGPVPEPEYLQ KLHKYLITRTERNVAVYAES ATYCLVKNAVKMFKDIGVFK ETKQKRVSVLELSSTFLPQC NRQKLLEYILSFVVL
Function Mitochondrial membrane protein that catalyzes the essential first step of biosynthesis of glycerolipids such as triglycerides, phosphatidic acids and lysophosphatidic acids. Esterifies acyl-group from acyl-coenzyme A (acyl-CoA) to the sn-1 position of glycerol-3-phosphate, to produce lysophosphatidic acid. Has a narrow hydrophobic binding cleft that selects for a linear acyl chain. Catalytic activity is higher for substrates with a 16-carbon acyl chain.
Protein Name Glycerol-3-Phosphate Acyltransferase 1 - Mitochondrial
Gpat-1
Database Links Reactome: R-HSA-1483166
Reactome: R-HSA-2426168
Reactome: R-HSA-75109
Reactome: R-HSA-8931987
Reactome: R-HSA-9018519
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Associated With The Mitochondrion Outer Membrane Of Hepatic Cells Via A Patch Of Basic Residues
Alternative Antibody Names Anti-Glycerol-3-Phosphate Acyltransferase 1 - Mitochondrial antibody
Anti-Gpat-1 antibody
Anti-GPAM antibody
Anti-GPAT1 antibody
Anti-KIAA1560 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance