| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase (623-722) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4144RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GNE |
| Gene ID: |
10020 |
| Uniprot ID: |
GLCNE_HUMAN |
| Immunogen Region: |
623-722 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 623-722 of human GNE (Q9Y223). |
| Immunogen Sequence: |
GALHLIQAAKLGNAKAQSIL RTAGTALGLGVVNILHTMNP SLVILSGVLASHYIHIVKDV IRQQALSSVQDVDVVVSDLV DPALLGAASMVLDYTTRRIY |
| Tissue Specificity | Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon. |
| Post Translational Modifications | Phosphorylated by PKC. |
| Function | Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. |
| Protein Name | Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine KinaseUdp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-EpimeraseHydrolyzingUdp-Glcnac-2-EpimeraseUridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine KinaseManac Kinase |
| Database Links | Reactome: R-HSA-4085001Reactome: R-HSA-4085011 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase antibodyAnti-Udp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-Epimerase antibodyAnti-Hydrolyzing antibodyAnti-Udp-Glcnac-2-Epimerase antibodyAnti-Uridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine Kinase antibodyAnti-Manac Kinase antibodyAnti-GNE antibodyAnti-GLCNE antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BRD4 knockout (KO) HeLa cells, using [KO Validated] BRD4 Rabbit polyclonal antibody (STJ11101789) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/90704/360807/STJ11101789_1__96955.1713125533.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BRD4 knockout (KO) HeLa cells, using [KO Validated] BRD4 Rabbit polyclonal antibody (STJ11101789) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western blot analysis of lysates from wild type (WT) and BRD4 knockout (KO) HeLa cells, using [KO Validated] BRD4 Rabbit polyclonal antibody (STJ11101529) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/90442/359824/STJ11101529_1__04420.1713124358.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BRD4 knockout (KO) HeLa cells, using [KO Validated] BRD4 Rabbit polyclonal antibody (STJ11101529) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.")