Anti-GNAI3 antibody (31-80 aa) (STJ93457)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GNAI3 at the amino acid range 31-80
General Information
| Short Description | Rabbit polyclonal anti-Guanine nucleotide-binding protein Gi subunit alpha-3 alpha-3 (31-80 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GNAI3 |
| Gene ID | 2773 |
| Uniprot ID | GNAI3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GNAI3 at the amino acid range 31-80 |
| Immunogen Region | 31-80 aa |
| Specificity | G Alpha i-3 Polyclonal Antibody detects endogenous levels of G Alpha i-3 protein. |
Additional Info
| Function | Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. The active GTP-bound form activates the calcium permeant TRPC5 ion channels. |
| Protein Name | Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3G(I Alpha-3 |
| Database Links | Reactome: R-HSA-170670Reactome: R-HSA-392170Reactome: R-HSA-418555Reactome: R-HSA-418594Reactome: R-HSA-418597Reactome: R-HSA-9009391Reactome: R-HSA-9634597Reactome: R-HSA-9660821 |
| Cellular Localisation | CytoplasmCell MembraneLipid-AnchorCytoskeletonMicrotubule Organizing CenterCentrosomeLocalizes In The Centrosomes Of Interphase And Mitotic CellsDetected At The Cleavage Furrow And/Or The Midbody |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3 antibodyAnti-G(I Alpha-3 antibodyAnti-GNAI3 antibody |
Information sourced from Uniprot.org