• Immunofluorescence analysis of NIH-3T3 cells using GNAI2 Rabbit polyclonal antibody (STJ116758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using GNAI2 Rabbit polyclonal antibody (STJ116758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GNAI2 Rabbit polyclonal antibody (STJ116758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GNAI2 Rabbit polyclonal antibody (STJ116758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GNAI2 antibody (1-355) (STJ116758)

SKU:
STJ116758

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNAI2 (1-355) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNAI2
Gene ID: 2771
Uniprot ID: GNAI2_HUMAN
Immunogen Region: 1-355
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-355 of human GNAI2 (NP_002061.1).
Immunogen Sequence: MGCTVSAEDKAAAERSKMID KNLREDGEKAAREVKLLLLG AGESGKSTIVKQMKIIHEDG YSEEECRQYRAVVYSNTIQS IMAIVKAMGNLQIDFADPSR ADDARQLFALSCTAEEQGVL PDDLSGVIRRLWADHGVQAC FGRSREYQLNDSAAYYLNDL ERIAQSDYIPTQQDVLRTRV KTTGIVETHFTFKDLHFKMF DVGGQRSERKKWIHCFEGVT AIIFCVALSAYDLVLAEDE
Post Translational Modifications (Microbial infection) Deamidated at Gln-205 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. Isoform sGi2: Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool.
Protein Name Guanine Nucleotide-Binding Protein G(I Subunit Alpha-2
Adenylate Cyclase-Inhibiting G Alpha Protein
Database Links Reactome: R-HSA-170670
Reactome: R-HSA-392170
Reactome: R-HSA-400042
Reactome: R-HSA-418555
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Reactome: R-HSA-422356
Reactome: R-HSA-9009391
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cell Membrane
Membrane
Lipid-Anchor
Localizes In The Centrosomes Of Interphase And Mitotic Cells
Detected At The Cleavage Furrow And/Or The Midbody
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(I Subunit Alpha-2 antibody
Anti-Adenylate Cyclase-Inhibiting G Alpha Protein antibody
Anti-GNAI2 antibody
Anti-GNAI2B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance