| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Guanine nucleotide-binding protein Gi subunit alpha-1 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GNAI1 |
| Gene ID: |
2770 |
| Uniprot ID: |
GNAI1_HUMAN |
| Specificity: |
GNAI1 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from part of the human protein |
| Function | Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis. Required for cortical dynein-dynactin complex recruitment during metaphase. |
| Protein Name | Guanine Nucleotide-Binding Protein G(I Subunit Alpha-1Adenylate Cyclase-Inhibiting G Alpha Protein |
| Database Links | Reactome: R-HSA-170670Reactome: R-HSA-392170Reactome: R-HSA-400042Reactome: R-HSA-418555Reactome: R-HSA-418594Reactome: R-HSA-418597Reactome: R-HSA-422356Reactome: R-HSA-9009391Reactome: R-HSA-9634597Reactome: R-HSA-9660821 |
| Cellular Localisation | NucleusCytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic SideCytoskeletonMicrotubule Organizing CenterCentrosomeCell CortexMembraneLipid-AnchorLocalizes In The Centrosomes Of Interphase And Mitotic CellsBut Not In Centrosomes During CytokinesisDetected At The Cleavage Furrow Or The MidbodyLocalized At The Plasma Membrane Throughout MitosisColocalizes With Ric8a And Rgs14 At The Plasma Membrane |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein G(I Subunit Alpha-1 antibodyAnti-Adenylate Cyclase-Inhibiting G Alpha Protein antibodyAnti-GNAI1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
