Anti-GNA12 antibody (285-381) (STJ115691)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GNA12 (285-381) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GNA12 |
| Gene ID | 2768 |
| Uniprot ID | GNA12_HUMAN |
| Immunogen Region | 285-381 |
| Immunogen Sequence | LFFNVSIILFLNKMDLLVEK VKTVSIKKHFPDFRGDPHRL EDVQRYLVQCFDRKRRNRSK PLFHHFTTAIDTENVRFVFH AVKDTILQENLKDIMLQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 285-381 of human GNA12 (NP_031379.2). |
Additional Info
| Function | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins. Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating pro-inflammatory cytokine production. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. Together with NAPA promotes CDH5 localization to plasma membrane. May play a role in the control of cell migration through the TOR signaling cascade. |
| Protein Name | Guanine Nucleotide-Binding Protein Subunit Alpha-12G Alpha-12G-Protein Subunit Alpha-12 |
| Database Links | Reactome: R-HSA-416482Reactome: R-HSA-456926 |
| Cellular Localisation | Cell MembraneLipid-AnchorLateral Cell MembraneCytoplasmCdh1 Enhances Cell Membrane Localization |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-12 antibodyAnti-G Alpha-12 antibodyAnti-G-Protein Subunit Alpha-12 antibodyAnti-GNA12 antibody |
Information sourced from Uniprot.org