| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GNA12 (285-381) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GNA12 |
| Gene ID: |
2768 |
| Uniprot ID: |
GNA12_HUMAN |
| Immunogen Region: |
285-381 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 285-381 of human GNA12 (NP_031379.2). |
| Immunogen Sequence: |
LFFNVSIILFLNKMDLLVEK VKTVSIKKHFPDFRGDPHRL EDVQRYLVQCFDRKRRNRSK PLFHHFTTAIDTENVRFVFH AVKDTILQENLKDIMLQ |
| Function | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins. Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating pro-inflammatory cytokine production. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. Together with NAPA promotes CDH5 localization to plasma membrane. May play a role in the control of cell migration through the TOR signaling cascade. |
| Protein Name | Guanine Nucleotide-Binding Protein Subunit Alpha-12G Alpha-12G-Protein Subunit Alpha-12 |
| Database Links | Reactome: R-HSA-416482Reactome: R-HSA-456926 |
| Cellular Localisation | Cell MembraneLipid-AnchorLateral Cell MembraneCytoplasmCdh1 Enhances Cell Membrane Localization |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-12 antibodyAnti-G Alpha-12 antibodyAnti-G-Protein Subunit Alpha-12 antibodyAnti-GNA12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
