• Immunofluorescence analysis of NIH/3T3 cells using GNA11 Rabbit polyclonal antibody (STJ11103920) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GNA11 Rabbit polyclonal antibody (STJ11103920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GNA11 antibody (250-350) (STJ11103920)

SKU:
STJ11103920

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Guanine Nucleotide-Binding Protein Subunit Alpha-11 (250-350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNA11
Gene ID: 2767
Uniprot ID: GNA11_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human GNA11 (NP_002058.2).
Immunogen Sequence: ESKALFRTIITYPWFQNSSV ILFLNKKDLLEDKILYSHLV DYFPEFDGPQRDAQAAREFI LKMFVDLNPDSDKIIYSHFT CATDTENIRFVFAAVKDTIL Q
Tissue Specificity Expressed in testis.
Post Translational Modifications (Microbial infection) Deamidated at Gln-209 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C. Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs). Together with GNAQ, required for heart development.
Protein Name Guanine Nucleotide-Binding Protein Subunit Alpha-11
G Alpha-11
G-Protein Subunit Alpha-11
Guanine Nucleotide-Binding Protein G(Y Subunit Alpha
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-202040
Reactome: R-HSA-399997
Reactome: R-HSA-416476
Reactome: R-HSA-418592
Reactome: R-HSA-428930
Reactome: R-HSA-434316
Reactome: R-HSA-456926
Reactome: R-HSA-6814122
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasm
In Testicular Cells
Expressed Exclusively In The Cytoplasm
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-11 antibody
Anti-G Alpha-11 antibody
Anti-G-Protein Subunit Alpha-11 antibody
Anti-Guanine Nucleotide-Binding Protein G(Y Subunit Alpha antibody
Anti-GNA11 antibody
Anti-GA11 antibody

Information sourced from Uniprot.org

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