| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-GM2A (164-175) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 24kDa band was observed in Rat Kidney lysates (calculated MW of 21.5kDa according to NP_758838.2 ). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | GM2A |
| Gene ID: | 2760 |
| Uniprot ID: | SAP3_HUMAN |
| Immunogen Region: | 164-175 |
| Accession Number: | NP_000396.2 |
| Specificity: | This antibody is expected to recognize isoform 1 (NP_000396.2) only. |
| Immunogen Sequence: | TTGNYRIESVLS |
| Function | The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity. Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Has cholesterol transfer activity. |
| Protein Name | Ganglioside Gm2 ActivatorCerebroside Sulfate Activator ProteinGm2-ApSphingolipid Activator Protein 3Sap-3 Cleaved Into - Ganglioside Gm2 Activator Isoform Short |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-9840310 |
| Cellular Localisation | Lysosome |
| Alternative Antibody Names | Anti-Ganglioside Gm2 Activator antibodyAnti-Cerebroside Sulfate Activator Protein antibodyAnti-Gm2-Ap antibodyAnti-Sphingolipid Activator Protein 3 antibodyAnti-Sap-3 Cleaved Into - Ganglioside Gm2 Activator Isoform Short antibodyAnti-GM2A antibody |
Information sourced from Uniprot.org
