| Host: |
Mouse |
| Applications: |
IHC-P |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Glutamine Synthetase (50-250aa) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
ZM377 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b/Kappa |
| Formulation: |
Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: |
Affinity purified |
| Dilution Range: |
1:100-200 |
| Storage Instruction: |
Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Gene Symbol: |
GLUL |
| Gene ID: |
2752 |
| Uniprot ID: |
GLNA_HUMAN |
| Immunogen Region: |
50-250aa |
| Specificity: |
Positive control: Normal liver |
| Immunogen: |
Recombinant fragment (around aa 50-250) of human GLUL protein |
| Tissue Specificity | Expressed in endothelial cells. |
| Post Translational Modifications | Acetylated by EP300/p300.acetylation is stimulated by increased glutamine levels and promotes ubiquitin-mediated proteasomal degradation. Palmitoylated.undergoes autopalmitoylation. Ubiquitinated by ZNRF1. Ubiquitinated by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(CRBN), leading to proteasomal degradation. |
| Function | Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia. Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis. Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2. |
| Protein Name | Glutamine SynthetaseGsGlutamate--Ammonia LigasePalmitoyltransferase Glul |
| Database Links | Reactome: R-HSA-210455Reactome: R-HSA-8964539 |
| Cellular Localisation | CytoplasmCytosolMicrosomeMitochondrionCell MembraneLipid-AnchorMainly Localizes In The CytosolWith A Fraction Associated With The Cell Membrane |
| Alternative Antibody Names | Anti-Glutamine Synthetase antibodyAnti-Gs antibodyAnti-Glutamate--Ammonia Ligase antibodyAnti-Palmitoyltransferase Glul antibodyAnti-GLUL antibodyAnti-GLNS antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
