Anti-Glutamine Synthetase antibody (1-373aa) [AT8D7] (STJA0041847)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenRecombinant human GLUL (1-373aa) purified from E. coli
General Information
| Short Description | Mouse monoclonal anti-Glutamine Synthetase (1-373aa) for use in ELISA, WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IF |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT8D7 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-A affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GLUL |
| Gene ID | 2752 |
| Uniprot ID | GLNA_HUMAN |
| Accession Number | NP_001028216 |
| Immunogen | Recombinant human GLUL (1-373aa) purified from E. coli |
| Immunogen Region | 1-373aa |
Additional Info
| Tissue Specificity | Expressed in endothelial cells. |
| Post Translational Modifications | Acetylated by EP300/p300.acetylation is stimulated by increased glutamine levels and promotes ubiquitin-mediated proteasomal degradation. Palmitoylated.undergoes autopalmitoylation. Ubiquitinated by ZNRF1. Ubiquitinated by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(CRBN), leading to proteasomal degradation. |
| Function | Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia. Plays a key role in ammonium detoxification during erythropoiesis: the glutamine synthetase activity is required to remove ammonium generated by porphobilinogen deaminase (HMBS) during heme biosynthesis to prevent ammonium accumulation and oxidative stress. Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis. Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2. |
| Protein Name | Glutamine SynthetaseGsGlutamate--Ammonia LigasePalmitoyltransferase Glul |
| Database Links | Reactome: R-HSA-210455Reactome: R-HSA-8964539 |
| Cellular Localisation | CytoplasmCytosolMicrosomeMitochondrionCell MembraneLipid-AnchorMainly Localizes In The CytosolWith A Fraction Associated With The Cell Membrane |
| Alternative Antibody Names | Anti-Glutamine Synthetase antibodyAnti-Gs antibodyAnti-Glutamate--Ammonia Ligase antibodyAnti-Palmitoyltransferase Glul antibodyAnti-GLUL antibodyAnti-GLNS antibody |
Information sourced from Uniprot.org