| Host: | Rabbit |
| Applications: | CM/ELISA/ICC/IF/IHC/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-GLUT4 (450-509) is suitable for use in Confocal Microscopy, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5 µg/µl |
| Dilution Range: | WB: 1:1, 000 DB: 1:4, 000 ELISA: 1:4, 000 IP: 1:200 IHC: 1:200 ICC: 1:200 IF: 1:200 CFM: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Slc2a4 |
| Gene ID: | 20528 |
| Uniprot ID: | GLUT4_MOUSE |
| Immunogen Region: | 450-509 |
| Immunogen: | Synthetic peptide taken within amino acid region 450-509 on mouse Solute carrier family 2, facilitated glucose transporter member 4 protein. |
| Tissue Specificity | Expressed in skeletal and cardiac muscles. Expressed in brown and white adipose tissues. |
| Post Translational Modifications | Sumoylated. Palmitoylated. Palmitoylation by ZDHHC7 controls the insulin-dependent translocation of GLUT4 to the plasma membrane. |
| Function | Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell. |
| Protein Name | Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 Gt2 Glucose Transporter Type 4 - Insulin-Responsive Glut-4 |
| Database Links | Reactome: R-MMU-189200 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Endomembrane System Cytoplasm Perinuclear Region Localizes Primarily To The Perinuclear Region Undergoing Continued Recycling To The Plasma Membrane Where It Is Rapidly Reinternalized The Dileucine Internalization Motif Is Critical For Intracellular Sequestration Insulin Stimulation Induces Translocation To The Cell Membrane |
| Alternative Antibody Names | Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 antibody Anti-Gt2 antibody Anti-Glucose Transporter Type 4 - Insulin-Responsive antibody Anti-Glut-4 antibody Anti-Slc2a4 antibody Anti-Glut4 antibody |
Information sourced from Uniprot.org