• Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ27390) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ27390) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Glutamine Synthetase (GLUL) in paraffin-embedded rat liver using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ27390) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Glutamine Synthetase (GLUL) in paraffin-embedded human liver using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ27390) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Glutamine Synthetase (GLUL) in paraffin-embedded human esophageal using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ27390) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ27390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GLUL antibody (1-373) (STJ27390)

SKU:
STJ27390

€161.88 - €799.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLUL (1-373) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLUL
Gene ID: 2752
Uniprot ID: GLNA_HUMAN
Immunogen Region: 1-373
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human Glutamine Synthetase (GLUL) (NP_002056.2).
Immunogen Sequence: MTTSASSHLNKGIKQVYMSL PQGEKVQAMYIWIDGTGEGL RCKTRTLDSEPKCVEELPEW NFDGSSTLQSEGSNSDMYLV PAAMFRDPFRKDPNKLVLCE VFKYNRRPAETNLRHTCKRI MDMVSNQHPWFGMEQEYTLM GTDGHPFGWPSNGFPGPQGP YYCGVGADRAYGRDIVEAHY RACLYAGVKIAGTNAEVMPA QWEFQIGPCEGISMGDHLWV ARFILHRVCEDFGVIATFD
Tissue Specificity Expressed in endothelial cells.
Post Translational Modifications Palmitoylated.undergoes autopalmitoylation. Ubiquitinated by ZNRF1.
Function Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia. Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis.
Protein Name Glutamine Synthetase
Gs
Glutamate--Ammonia Ligase
Palmitoyltransferase Glul
Database Links Reactome: R-HSA-210455
Reactome: R-HSA-8964539
Cellular Localisation Cytoplasm
Cytosol
Microsome
Mitochondrion
Cell Membrane
Lipid-Anchor
Mainly Localizes In The Cytosol
With A Fraction Associated With The Cell Membrane
Alternative Antibody Names Anti-Glutamine Synthetase antibody
Anti-Gs antibody
Anti-Glutamate--Ammonia Ligase antibody
Anti-Palmitoyltransferase Glul antibody
Anti-GLUL antibody
Anti-GLNS antibody

Information sourced from Uniprot.org

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