Anti-Glucokinase antibody [R04-7V9] (STJA0037270)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Glucokinase
General Information
| Short Description | Rabbit monoclonal anti-Glucokinase for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-7V9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GCK |
| Gene ID | 2645 |
| Uniprot ID | HXK4_HUMAN |
| Immunogen | A synthesized peptide derived from human Glucokinase |
Additional Info
| Function | Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. |
| Protein Name | Hexokinase-4Hk4GlucokinaseHexokinase Type IvHk IvHexokinase-D |
| Database Links | Reactome: R-HSA-170822Reactome: R-HSA-210745Reactome: R-HSA-5619073Reactome: R-HSA-5619107Reactome: R-HSA-70171Reactome: R-HSA-9615017 |
| Cellular Localisation | CytoplasmNucleusMitochondrionUnder Low Glucose ConcentrationsGck Associates With Gckr And The Inactive Complex Is Recruited To The Hepatocyte Nucleus |
| Alternative Antibody Names | Anti-Hexokinase-4 antibodyAnti-Hk4 antibodyAnti-Glucokinase antibodyAnti-Hexokinase Type Iv antibodyAnti-Hk Iv antibodyAnti-Hexokinase-D antibodyAnti-GCK antibody |
Information sourced from Uniprot.org