Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GLS antibody (162-212 aa) (STJ194432)

SKU:
STJ194432
£49.00 - £261.00
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Short Description :Rabbit polyclonal anti-Glutaminase kidney isoform, mitochondrial (162-212 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:1 mg/mL
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution Range:WB 1:500-2000
Formulation:Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:GLS
Gene ID:2744
Uniprot ID:GLSK_HUMAN
Immunogen:Synthesized peptide derived from the human GLSK at the amino acid range 162-212
Immunogen Region:162-212 aa
Specificity:This antibody detects endogenous levels of GLSK at Human/Mouse/Rat
Post Translational Modifications Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.
Function Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. Isoform 2: Lacks catalytic activity.
Protein Name Glutaminase Kidney Isoform - Mitochondrial
Gls
K-Glutaminase
L-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain
Database Links Reactome: R-HSA-210500
Reactome: R-HSA-5628897
Reactome: R-HSA-8964539
Cellular Localisation Isoform 1: Mitochondrion
Cytoplasm
Cytosol
The 74-Kda Cytosolic Precursor Is Translocated Into The Mitochondria And Processed Via A 72-Kda Intermediate To Yield The Mature 68- And 65-Kda Subunits
Isoform 3: Mitochondrion
Glutaminase Kidney Isoform
Mitochondrial 68 Kda Chain: Mitochondrion Matrix
Produced By The Proteolytic Processing Of The 74-Kda Cytosolic Precursor
Mitochondrial 65 Kda Chain: Mitochondrion Matrix
Alternative Antibody Names Anti-Glutaminase Kidney Isoform - Mitochondrial antibody
Anti-Gls antibody
Anti-K-Glutaminase antibody
Anti-L-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain antibody
Anti-GLS antibody
Anti-GLS1 antibody
Anti-KIAA0838 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ195337 Anti-RPH3AL antibody (162-212 aa) (STJ195337) Rabbit Polyclonal Human/Mouse/Rat WB
STJ194939 Anti-TLCD1 antibody (162-212 aa) (STJ194939) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA/IHC
STJ193894 Anti-TRIM2 antibody (162-212 aa) (STJ193894) Rabbit Polyclonal Human/Mouse/Rat WB
STJ195121 Anti-SLC25A24 antibody (162-212 aa) (STJ195121) Rabbit Polyclonal Human/Mouse WB/ELISA/IHC
STJ194064 Anti-PPA1 antibody (162-212 aa) (STJ194064) Rabbit Polyclonal Human/Mouse WB

Related Products

!