| Host: |
Rabbit |
| Applications: |
IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GLI3 (681-788) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GLI3 |
| Gene ID: |
2737 |
| Uniprot ID: |
GLI3_HUMAN |
| Immunogen Region: |
681-788 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 681-788 of human GLI3 (NP_000159.3). |
| Immunogen Sequence: |
NTTSKREECLQVKTVKAEKP MTSQPSPGGQSSCSSQQSPI SNYSNSGLELPLTDGGSIGD LSAIDETPIMDSTISTATTA LALQARRNPAGTKWMEHVKL ERLKQVNG |
| Tissue Specificity | Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. |
| Post Translational Modifications | Phosphorylated on multiple sites by protein kinase A (PKA) and phosphorylation by PKA primes further phosphorylation by CK1 and GSK3. Phosphorylated by DYRK2 (in vitro). Phosphorylation is essential for its proteolytic processing. Transcriptional repressor GLI3R, a C-terminally truncated form, is generated from the full-length GLI3 protein (GLI3FL/GLI3-190) through proteolytic processing. This process requires PKA-primed phosphorylation of GLI3, ubiquitination of GLI3 and the presence of BTRC. GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. GLI3R formation leads to its dissociation from SUFU, allowing it to translocate into the nucleus, and repress Hh target genes. When Hh signaling is initiated, SUFU dissociates from GLI3FL and this has two consequences. First, GLI3R production is halted. Second, free GLI3FL translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Phosphorylated in vitro by ULK3. |
| Function | Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. |
| Protein Name | Transcriptional Activator Gli3Gli3 Form Of 190 KdaGli3-190Gli3 Full-Length ProteinGli3fl Cleaved Into - Transcriptional Repressor Gli3rGli3 C-Terminally Truncated FormGli3 Form Of 83 KdaGli3-83 |
| Database Links | Reactome: R-HSA-5610785Reactome: R-HSA-5610787Reactome: R-HSA-5632684Reactome: R-HSA-5635851Reactome: R-HSA-8940973 |
| Cellular Localisation | NucleusCytoplasmCell ProjectionCiliumGli3fl Is Localized Predominantly In The Cytoplasm While Gli3r Resides Mainly In The NucleusCiliary Accumulation Requires The Presence Of Kif7 And SmoTranslocation To The Nucleus Is Promoted By Interaction With Zic1 |
| Alternative Antibody Names | Anti-Transcriptional Activator Gli3 antibodyAnti-Gli3 Form Of 190 Kda antibodyAnti-Gli3-190 antibodyAnti-Gli3 Full-Length Protein antibodyAnti-Gli3fl Cleaved Into - Transcriptional Repressor Gli3r antibodyAnti-Gli3 C-Terminally Truncated Form antibodyAnti-Gli3 Form Of 83 Kda antibodyAnti-Gli3-83 antibodyAnti-GLI3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
