• Immunohistochemistry analysis of Glycerol kinase (GK) in paraffin-embedded human liver using Glycerol kinase (GK) Rabbit polyclonal antibody (STJ11100926) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Glycerol kinase (GK) in paraffin-embedded human esophageal cancer using Glycerol kinase (GK) Rabbit polyclonal antibody (STJ11100926) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Glycerol kinase (GK) Rabbit polyclonal antibody (STJ11100926) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-GK antibody (422-559) (STJ11100926)

SKU:
STJ11100926

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GK (422-559) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GK
Gene ID: 2710
Uniprot ID: GLPK_HUMAN
Immunogen Region: 422-559
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 422-559 of Glycerol kinase (GK) (NP_001191948.1).
Immunogen Sequence: GIPLSHLQVDGGMTSNKILM QLQADILYIPVVKPSMPETT ALGAAMAAGAAEGVGVWSLE PEDLSAVTMERFEPQINAEE SEIRYSTWKKAVMKSMGWVT TQSPESGDPSIFCSLPLGFF IVSSMVMLIGARYISGIP
Tissue Specificity Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
Function Key enzyme in the regulation of glycerol uptake and metabolism.
Protein Name Glycerol Kinase
Gk
Glycerokinase
Atp -Glycerol 3-Phosphotransferase
Database Links Reactome: R-HSA-75109
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
In Sperm And Fetal Tissues
The Majority Of The Enzyme Is Bound To Mitochondria
But In Adult Tissues
Such As Liver Found In The Cytoplasm
Alternative Antibody Names Anti-Glycerol Kinase antibody
Anti-Gk antibody
Anti-Glycerokinase antibody
Anti-Atp -Glycerol 3-Phosphotransferase antibody
Anti-GK antibody

Information sourced from Uniprot.org

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